streptavidin treatment (Agilent technologies)
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streptavidin treatment
Streptavidin Treatment, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 25408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+treatment/Streptavidin/pmc01242152-123-20-9
Average 99 stars, based on 25408 article reviews
Streptavidin Treatment, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 25408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+treatment/Streptavidin/pmc01242152-123-20-9
Average 99 stars, based on 25408 article reviews
streptavidin treatment - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Clinicopathologic Analysis of Breast Cancers with PIK3CA Mutations in Japanese Women Article Snippet: After several washes in TBS-T, antigen retrieval was done by heating the samples in 10 mmol/L citrate buffer (pH 6.0) at 95jC for 40 min. After blocking serum (DAKO Diagnostics, Mississauga, Ontario, Canada) for 30 min, the samples were incubated with a polyclonal rabbit anti–pAKT (Ser473) antibody (1:100 dilution; Cell Signaling Technologies, Beverly, MA) or with a polyclonal anti–phospho-S6 ribosomal protein (1:50 dilution; Cell Signaling Technologies) at 4jC overnight. .. We then used the LSAB+ System (DAKO), which involved incubation with Article Title: Celecoxib analogues disrupt Akt signaling, which is commonly activated in primary breast tumours Article Snippet: The primary antibody (Phospho-AKT S473 IHC Specific; CST) was diluted 1:250 with a 1% bovine serum albumin solution, applied to the sides and incubated overnight at 4°C. .. For signal amplification, we then used the LSAB+ System (DAKO), which involved incubation with a biotinylated secondary antibody followed by Article Title: Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells. Article Snippet: The primary antibody (Phospho-AKT S473 IHC Specific, Cell Signaling Technologies (CST), Beverly, MA, USA) was diluted 1 : 250 with a 1% BSA solution, applied to the slides, and incubated overnight at 41C. .. For signal amplification, we used the LSABþ System (DAKO), which involved incubation with a biotinylated secondary antibody followed by Amplification:Article Title: Clinicopathologic Analysis of Breast Cancers with PIK3CA Mutations in Japanese Women Article Snippet: After several washes in TBS-T, antigen retrieval was done by heating the samples in 10 mmol/L citrate buffer (pH 6.0) at 95jC for 40 min. After blocking serum (DAKO Diagnostics, Mississauga, Ontario, Canada) for 30 min, the samples were incubated with a polyclonal rabbit anti–pAKT (Ser473) antibody (1:100 dilution; Cell Signaling Technologies, Beverly, MA) or with a polyclonal anti–phospho-S6 ribosomal protein (1:50 dilution; Cell Signaling Technologies) at 4jC overnight. .. We then used the LSAB+ System (DAKO), which involved incubation with Article Title: Celecoxib analogues disrupt Akt signaling, which is commonly activated in primary breast tumours Article Snippet: The primary antibody (Phospho-AKT S473 IHC Specific; CST) was diluted 1:250 with a 1% bovine serum albumin solution, applied to the sides and incubated overnight at 4°C. .. For signal amplification, we then used the LSAB+ System (DAKO), which involved incubation with a biotinylated secondary antibody followed by Article Title: Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells. Article Snippet: The primary antibody (Phospho-AKT S473 IHC Specific, Cell Signaling Technologies (CST), Beverly, MA, USA) was diluted 1 : 250 with a 1% BSA solution, applied to the slides, and incubated overnight at 41C. .. For signal amplification, we used the LSABþ System (DAKO), which involved incubation with a biotinylated secondary antibody followed by Avidin-Biotin Assay:Article Title: Clinicopathologic Analysis of Breast Cancers with PIK3CA Mutations in Japanese Women Article Snippet: After several washes in TBS-T, antigen retrieval was done by heating the samples in 10 mmol/L citrate buffer (pH 6.0) at 95jC for 40 min. After blocking serum (DAKO Diagnostics, Mississauga, Ontario, Canada) for 30 min, the samples were incubated with a polyclonal rabbit anti–pAKT (Ser473) antibody (1:100 dilution; Cell Signaling Technologies, Beverly, MA) or with a polyclonal anti–phospho-S6 ribosomal protein (1:50 dilution; Cell Signaling Technologies) at 4jC overnight. .. We then used the LSAB+ System (DAKO), which involved incubation with |